Explore how Dextramer® reagents support the development of effective cell therapies.
CAR Dextramer® hCD19 for Sensitive Detection and Characterization of CD19-Specific CAR Cells
Discover the power of CAR Dextramer® reagents for the sensitive detection and characterization of CAR-positive cells using flow cytometry.
CAR Dextramer® hCD19
CD19 is a key target for CAR T cell therapy, a groundbreaking treatment that has transformed cancer immunotherapy by enabling precise targeting of B-cell malignancies. Accurate detection of CD19-specific CAR T cells is essential for both therapy development and patient monitoring.
Through multimerization of high-quality CD19 proteins and fluorochrome molecules, CAR Dextramer® hCD19 provides researchers and clinicians with a highly precise and sensitive tool for CAR cell detection.
Read the Blog: “Avidity can make all the difference in detecting CAR-engineered cells”.
Catalog Number |
Product Name/Description |
Test Size |
Dye |
CT001 | CAR Dextramer®, hCD19 | 50 tests | PE |
Superior CAR Cell Detection with CAR Dextramer® hCD19
CAR Dextramer® hCD19 exhibits a high mean fluorescence intensity (MFI), resulting in superior staining brightness and enhanced detection sensitivity. Optimize your CD19 CAR T cell analysis with CAR Dextramer® hCD19.

Primary human CD19 CAR-T cells stained with PE-labelled CAR Dextramer® hCD19 or a commercially available anti-FMC63 idiotype antibody. Control Dextramer® was used as negative control.
Want to see more results?
Our collaborators BioNTech SE have compared four CAR detection reagents across different constructs and expression levels.
- The Results? CAR Dextramer® CD19 delivered strong detection in half the time
- CAR Dextramer® CD19 enabled superior sensitivity for low-expressing CAR constructs
- CAR Dextramer® bypasses scFv limitations like FMC63-only antibodies
Dive into the performance data in this blog post.
Enhanced CAR Cell Detection with Dextramer® Technology
- Optimized Reagent Design: Backed by over a decade of expertise in immune monitoring, our reagents are meticulously designed for maximum detection performance.
- Enhanced Sensitivity through Multimerization: Dextramer® enables multimerization of the target antigen, enhancing sensitivity in CAR detection, especially when CAR expression is low.
- Reliable Results: Provides consistent, high-quality results across experiments, ensuring reliable data for research and clinical applications.
- Simplified Staining Protocol: Our stable, optimized reagents allow for staining at room temperature with minimal wash steps, easing your workflow and minimizing the handling of your cell samples.
- Simultaneous phenotyping: Room-temperature staining enables the detection of CAR T cells alongside key surface markers, such as CCR7 and CD45, for efficient and comprehensive analysis.
Applications of CAR Dextramer® in CAR Cell Therapy
- Direct CAR cell detection
- Functional assessment of CAR-antigen interactions
- Enhanced sensitivity for cells with low CAR expression due to the high avidity of Dextramer® technology and multiple fluorophores
- Determine the % of CAR-positive cells
- Demonstrate that the infusion product meets the defined lot release criteria
- Monitor kinetics and persistence of infused CAR cells in patient blood samples
- Deeper characterization of target-specific CAR cells with gene and surface marker expression by single-cell multi-omics
FDA Guidance for CAR-T cell Therapy Products
The most recent guidance from the FDA regarding the development of CAR cell products includes recommendations for detecting and evaluating the CAR product.
Dextramer® reagents can assist you in adhering to this guidance.
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Dextramer® Controls
We recommend using negative controls with CAR Dextramer® reagents to accurately distinguish between background and antigen-specific staining, helping you optimize gating or sorting of positive cells.
One possible negative control Dextramer® reagent can be found here. Alternatively, we are happy to discuss other negative controls with you, or create a custom solution for you.
Related Resources
CAR Therapy Brochure
Learn how CAR Dextramer® enables superior detection and characterization of CAR cells.