Explore how Dextramer® reagents support the development of effective cell therapies.
Custom CAR Dextramer® for Rapid CAR Cell Detection and Characterization
Discover the power of CAR Dextramer® reagents for the rapid detection and characterization of CAR-positive cells using flow cytometry.
CAR Dextramer® Reagents
CAR Dextramer® reagents enable the reliable detection of CAR cells using a rapid one-step staining protocol.
Through multimerization of high-quality target proteins and fluorochrome molecules, CAR Dextramer® provides researchers and clinicians with a rapid and easy-to-use reagent for CAR cell detection. This antigen-based reagent serves two purposes: 1) to demonstrate the CAR’s presence on the cell surface, and 2) to demonstrate the ability of the CAR to bind to its target antigen.
Read the Blog: “Avidity can make all the difference in detecting CAR-engineered cells”.
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How does it work?
Our expert team designs and produces custom CAR Dextramer® reagents for you, based on your specific targets. The process takes only a matter of weeks and is optimized based on your specific targets and requirements.
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Related Products
CAR Dextramer® is already available as an off-the-shelf reagent for CAR cells targeting CD19, BCMA, and Mesothelin (MSLN).
Want to see the results for yourself?
Our collaborators BioNTech SE have compared four CAR detection reagents across different constructs and expression levels.
- The Results? CAR Dextramer® CD19 delivered strong detection in half the time
- CAR Dextramer® CD19 enabled superior sensitivity for low-expressing CAR constructs
- CAR Dextramer® bypasses scFv limitations like FMC63-only antibodies
Dive into the performance data in this blog post.
View performance data
Enhanced CAR Cell Detection with CAR Dextramer®
- Optimized Reagent Design: Backed by over a decade of expertise in immune monitoring, our reagents are meticulously designed for maximum detection performance.
- Antigen-Based Reagent: CAR Dextramer® directly demonstrates the CAR’s ability to bind to its target antigen - going beyond just presence to verify receptor functionality.
- Reliable Results: Provides consistent, high-quality results across experiments, ensuring reliable data for research and clinical applications.
- Simplified Staining Protocol: Our stable, optimized reagents allow for an easy one-step staining at room temperature with minimal wash steps, easing your workflow and minimizing the handling of your cell samples.
- Simultaneous phenotyping: Room-temperature staining enables the detection of CAR T cells alongside key surface markers, such as CCR7 and CD45, for efficient and comprehensive analysis.
Applications of CAR Dextramer® in CAR Cell Therapy
- Direct CAR cell detection
- Demonstrate that the CAR binds to its target antigen
- Determine the % of CAR-positive cells
- Demonstrate that the infusion product meets the defined lot release criteria
- Monitor kinetics and persistence of infused CAR cells in patient blood samples
- Deeper characterization of target-specific CAR cells with gene and surface marker expression by single-cell multi-omics
FDA Guidance for CAR-T cell Therapy Products
The most recent guidance from the FDA regarding the development of CAR cell products includes recommendations for detecting and evaluating the CAR product.
Dextramer® reagents can assist you in adhering to this guidance.
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Dextramer® Controls
We recommend using negative controls with CAR Dextramer® reagents to accurately distinguish between background and antigen-specific staining, helping you optimize gating or sorting of positive cells.
One possible negative control Dextramer® reagent can be found here. Alternatively, we are happy to discuss other negative controls with you, or create a custom solution for you.
Related Resources
CAR Therapy Brochure
Learn how CAR Dextramer® enables superior detection and characterization of CAR cells.