Reliable Identification of SARS-CoV-2-Specific T-Cell Responses

 

Explore T-Cell Immunity in COVID-19

A deeper understanding of virus-specific T-cell responses to COVID-19 is needed to evaluate vaccine candidates, explore long-term immunity, predict patients at risk, and even prepare for the next pandemic.

Dextramer® technology can help you monitor and characterize SARS-CoV-2- specific CD4+ and CD8+ T cells in blood, without missing rare or low-affinity cells, in an easy and reproducible way.​

Learn More about T Cells and COVID-19

Frequencies of SARS-CoV-2-specific CD8+ T cells detected in PBMCs at different time points from a vaccinated convalescent donor with a SARS-CoV-2 MHC I Panel of Non-Spike Epitopes.

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SARS-CoV-2 Dextramer® Reagents

All SARS-CoV-2 Dextramer® reagents are available for detection of antigen-specific T cells by in-situ staining, flow cytometry, NGS or single-cell multi-omics. All specificities listed below are also available as MHC Monomers.

MHC I or MHC II Dextramer® - for identification of antigen-specific T cells by flow cytometry

dCODE Dextramer® (HiT) - for bulk analysis of antigen-specific T cells by PCR and NGS

dCODE Dextramer® (10x) -  for single-cell multi-omic analysis of antigen-specific T cells to combine information on TCR recognition, TCR sequencing, and gene expression using 10x Chromium Single Cell analysis.

dCODE Dextramer® (RiO) - for single-cell multi-omic analysis of antigen-specific T cells to combine information on TCR recognition, TCR sequencing, and gene expression using BD RhapsodyTM Single-Cell Analysis.

MHC I or MHC II Monomers – for flexible investigation of antigen-specific T cells

                                          MHC Dextramer® Reagents

                                              dCODE Dextramer® Reagents

SARS-CoV-2 Dextramer® Panels

We provide SARS-CoV-2 Dextramer® reagents in panels targeting Spike or Non-Spike epitopes, each with a selection of validated T-cell epitopes.

Alternatively, select your own custom panel based on our selection of SARS-CoV-2 Dextramer® reagents, or order individual Dextramer® reagents.

The immunodominant SARS-CoV-2 specific T-cell epitopes listed below are known to be present in large populations, based on publications and in-house knowledge, enabling researchers to monitor T-cell immunity across COVID-19 variants.

SARS-CoV-2 MHC I Panel of Spike Epitopes

Allele

Peptide

Antigen

Delta

Omicron BA 2.12.1

Omicron BA.4

Omicron BA. 5

A*0101

LTDEMIAQY

S

 

 

 

 

A*0101

WTAGAAAYY

S

 

 

 

 

A*0201

YLQPRTFLL

S

 

 

 

 

A*0201

FIAGLIAIV

S

 

 

 

 

A*0301

KCYGVSPTK

S

 

 

 

 

A*0301

GVYFASTEK

S

 

GVYFASIEK

 

 

A*1101

RLFRKSNLK

S

 

 

 

 

A*1101

KCYGVSPTK

S

 

 

 

 

A*1101

GVYFASTEK

S

 

GVYFASIEK

 

 

A*2402

QYIKWPWYI

S

 

 

 

 

A*2402

NYNYLYRLF

S

NYNYRYRLF

 

NYNYRYRLF

NYNYRYRLF

B*0702

SPRRARSVA

S

SRRRARSVA

SHRRARSVA

SHRRARSVA

SHRRARSVA

B*0702

APHGVVFL

S

 

 

 

 

B*3501

QPTESIVRF

S

 

 

 

 

B*3501

LPFNDGVYF

S

 

 

 

 

B*3501

IPFAMQMAY

S

 

 

 

 

Spike-specific CD8+ T-cell epitopes in the SARS-CoV-2 Dextramer® Panels, and the conservation of these epitopes across the SARS-CoV-2 Delta (B.1.617.2) and Omicron (BA 2.12.1, BA. 4, BA. 5) variants. All epitopes are conserved in the reference strain (NC_045512). Mutations in the epitope are shown in red. Learn more in this Application Note.

SARS-CoV-2 MHC I Panel of Non-Spike Epitopes

Allele

Peptide

Antigen

Delta

Omicron

Omicron BA.4

Omicron BA. 5

A*0201

LLLDRLNQL

N

 

 

 

 

A*0301

KTFPPTEPK

N

 

 

 

 

A*1101

KTFPPTEPK

N

 

 

 

 

B*0702

SPRWYFYYL

N

 

 

 

 

A*0101

FTSDYYQLY

ORF3a

 

 

 

 

A*0201

LLYDANYFL

ORF3a

 

 

 

 

A*2402

VYFLQSINF

ORF3a

 

 

 

 

A*0101

TTDPSFLGRY

ORF1ab

TTDLSFLGRY

 

 

 

A*0201

ALWEIQQVV

ORF1ab

 

 

 

 

A*0301

VVYRGTTTYK

ORF1ab

 

 

 

 

A*1101

ASMPTTIAK

ORF1ab

 

 

 

 

A*2402

VYIGDPAQL

ORF1ab

 

 

 

 

B*0702

IPRRNVATL

ORF1ab

 

 

 

 

Non-Spike-specific CD8+ T-cell epitopes in the SARS-CoV-2 Dextramer® Panels, and the conservation of these epitopes across the SARS-CoV-2 Delta (B.1.617.2), and Omicron (BA 2.12.1, BA. 4, BA. 5) variants. Green = no mutations in epitope, Red = mutations in epitope. All epitopes are conserved in the reference strain (NC_045512). Mutations in the epitope are shown in red. Learn more in this Application Note.

Order your SARS-CoV-2 Panels

Please contact our application specialist if you would like to discuss your research.

Please include information on:

  • Your preferred epitope(s) and alleles for MHC Dextramer®, dCODE Dextramer®, or MHC Monomer
  • Your preferred reagent format: MHC Dextramer® or dCODE Dextramer®
  • Your preferred dye (for MHC Dextramer®): BV421, PE, APC, FITC, or None
  • Test size:
    o MHC Dextramer®, select between 50 or 150 tests
    o dCODE Dextramer®, select between 25, 50, or 150 tests
    o MHC Monomers select between 20 µg (~50 tests), 100 µg (~250 tests), or 200 µg (~500 tests)

Published Human COVID-19 T-Cell Epitopes

Download the list of epitopes in COVID-19 infected or vaccinated individuals

Published Mouse COVID-19 T-Cell Epitopes

Download the list of epitopes in COVID-19 infected genetically modified mice.

Want to Hear More About Our SARS-CoV-2 Solutions?

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BV421 is equivalent to Brilliant Violet™ 421, which is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by BD Innovation.