Reagents in accordance with ISO 13485 and 21 CFR 820
MHC I Dextramer® – Identify Antigen-Specific CD8+ T Cells
Capture the Complete Diversity of T Cells
MHC I binds and presents antigens from the cell cytosol to activate antigen-specific CD8+ T cells. This antigen-specific immune response is a key process in cancer, autoimmune disease, transplantation, infectious disease, and allergy. Thus, examining the full repertoire of CD8+ T cells, including those with low antigen affinity, is essential.
With an optimized number of MHC-peptide complexes and a choice of fluorophores (BV421, PE, APC, FITC, or none), MHC I Dextramer® reagents boost analysis sensitivity in flow cytometry beyond that achieved by other technologies.
READ MORE DETAILS IN OUR FAQs
MHC I Dextramer® detects low-affinity antigen-specific CD8+ T cells invisible to the corresponding tetramer (Dolton et al. 2014).
No Compromises in Performance
MHC I Dextramer® reagents are designed to meet strict quality and performance criteria, ensuring highly reliable research insights.
- Get the full picture of CD8+ T-cell responses, even the low-affinity ones.
- Ensure consistent, reproducible, and comparable results
- Expand the limits of your research
- Secure flexibility in your experiments
Discover Dextramer® Controls
“MHC Dextramers® are very helpful for us to label cells from immunization experiments to do target-specific cell sorting during the screening process.”
- Scientist, Pharmaceutical Company
Order MHC I Dextramer®
Use the webshop below to find and order your MHC I Dextramer® reagent online.
- If you can’t find a peptide specificity for your allele of choice in the webshop then you can specify and order your desired Dextramer® reagent with our custom tool below.
- In case you don’t find your MHC allele of interest or other special requests then please contact us. Our Custom Solutions and Services has a very good track record of taking on custom projects and producing new alleles.
- Alternatively, contact us specifying:
- Catalog number or desired MHC allele-peptide combination:
- Calculate the predicted MHC-peptide affinity using our prediction tool
- Consult our lists of available MHC alleles - Fluorophore (BV421, PE, APC, FITC, or none)
- Test size (50,150, 500, 1000 tests). One test is 10 µl reagent and sufficient to stain 1–3 x 106 lymphoid cells or 1–3 x 105 clonal antigen-specific T cells.
Mouse Cancer Models
We offer MHC I Dextramers for two major cancer models: B16 mouse melanoma model and MC38 mouse colorectal cancer model. These can be found in the webshop by filtering the category for these cancer models. We also offer MHC I Dextramers to detect T-cell responses to chicken ovalbumin (OVA) an antigen expressed by multiple syngeneic mouse cancer models to facilitate strong tumor immune responses. These can be found by filtering for the antigen OVA in the webshop below.
Can’t find what you’re looking for?
Our webshop does not include all products. Contact us or email [email protected] and we will search our larger internal database of products for you. We also provide custom alleles and specificities.
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For custom MHC-peptide combinations, a one-time entry fee will be required to evaluate each unique peptide's ability to bind to the requested MHC allele using standardized peptide binding assays. If the refolding process is unsuccessful, only the entry fee will be charged as we will not be able to deliver a product to you. The entry fee for custom MHC I Dextramer® reagents is 385 EUR / 445 USD.
BV421 is equivalent to Brilliant Violet™ 421, which is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by BD Innovation.