Single-Cell Evaluation Using a Panel of dCODE Dextramer® Reagents and the 10x Genomics Feature Barcode Protocol
Identifying disease-specific T-cell epitopes is key to developing novel immunotherapies and vaccines, while profiling them during a cellular immune response (e.g., tumor development or destruction) informs personalized immunotherapy.
Combining dCODE Dextramer® technology with the 10x Genomics Feature Barcode protocol allows coupling the T-cell receptor (TCR) specificity and sequence of antigen-specific T cells (ASTC) to the analysis of their transcriptional profile.
In this application note, discover how a panel of 50 dCODE Dextramer® specificities spanning 8 alleles was used to unravel T-cell antigen-specificity in a blood sample.
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After performing in-depth phenotyping of immune relevant cells, we:
Fig. 1: (A) t-SNE plot of the dCODE Dextramer®+ T cells, clustered and colored according to specificities. (B) HLA-DR and CCR7 staining corresponds to the bisection of the clusters.
