Explore how Dextramer® reagents support the development of effective cell therapies.
Custom CAR Dextramer® for Sensitive CAR Cell Detection and Characterization
Discover the power of CAR Dextramer® reagents for the sensitive detection and characterization of CAR-positive cells using flow cytometry.
CAR Dextramer® Reagents
CAR Dextramer® reagents enhance sensitivity, enabling the reliable detection of even lowly expressed CARs using a rapid one-step staining protocol.
Through multimerization of high-quality target proteins and fluorochromes, CAR Dextramer® provides researchers and clinicians with a highly precise and sensitive tool for CAR cell detection.
Read the Blog: “Avidity can make all the difference in detecting CAR-engineered cells”.
How does it work?
Our expert team designs custom CAR Dextramer® reagents for you, based on your specific targets.

Want to see the results for yourself?
Our collaborators BioNTech SE have compared four CAR detection reagents across different constructs and expression levels.
- The Results? CAR Dextramer® CD19 delivered strong detection in half the time
- CAR Dextramer® CD19 enabled superior sensitivity for low-expressing CAR constructs
- CAR Dextramer® bypasses scFv limitations like FMC63-only antibodies
Dive into the performance data in this blog post.
Enhanced CAR Cell Detection with CAR Dextramer®
- Optimized Reagent Design: Backed by over a decade of expertise in immune monitoring, our reagents are meticulously designed for maximum detection performance.
- Enhanced Sensitivity through Multimerization: Dextramer® enables multimerization of the target antigen, enhancing sensitivity in CAR detection, especially when CAR expression is low.
- Reliable Results: Provides consistent, high-quality results across experiments, ensuring reliable data for research and clinical applications.
- Simplified Staining Protocol: Our stable, optimized reagents allow for staining at room temperature with minimal wash steps, easing your workflow and minimizing the handling of your cell samples.
- Simultaneous phenotyping: Room-temperature staining enables the detection of CAR T cells alongside key surface markers, such as CCR7 and CD45, for efficient and comprehensive analysis.
Applications of CAR Dextramer® in CAR Cell Therapy
- Direct CAR cell detection
- Functional assessment of CAR-antigen interactions
- Enhanced sensitivity for cells with low CAR expression due to the high avidity of Dextramer® technology and multiple fluorophores
- Determine the % of CAR-positive cells
- Demonstrate that the infusion product meets the defined lot release criteria
- Monitor kinetics and persistence of infused CAR cells in patient blood samples
- Deeper characterization of target-specific CAR cells with gene and surface marker expression by single-cell multi-omics
FDA Guidance for CAR-T cell Therapy Products
The most recent guidance from the FDA regarding the development of CAR cell products includes recommendations for detecting and evaluating the CAR product.
Dextramer® reagents can assist you in adhering to this guidance.
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Dextramer® Controls
We recommend using negative controls with CAR Dextramer® reagents to accurately distinguish between background and antigen-specific staining, helping you optimize gating or sorting of positive cells.
One possible negative control Dextramer® reagent can be found here. Alternatively, we are happy to discuss other negative controls with you, or create a custom solution for you.
Related Resources
CAR Therapy Brochure
Learn how CAR Dextramer® enables superior detection and characterization of CAR cells.