Explore how Dextramer® reagents support the development of effective cell therapies.
Sensitive CAR Cell Detection and Quantification with Dextramer® Technology
Discover the power of CAR Dextramer® reagents for the sensitive detection and quantification of CAR-positive cells using flow cytometry.
CAR Dextramer® Reagents
CAR Dextramer® reagents enhance sensitivity, enabling the reliable detection of even lowly expressed CARs using a rapid one-step staining protocol.
Learn more about how Dextramer® technology can enhance your CAR cell detection.
CAR Dextramer® hCD19
CD19 is the key target for detecting and monitoring CD19-specific CAR cells, whether during development of a new therapy or after patient treatment.
With an optimized number of high-quality hCD19 protein and a PE fluorochrome, CD19 Dextramer® provides researchers and clinicians with a precise and sensitive tool for CAR cell detection.
Catalog Number |
Product Name/Description |
Test Size |
Dye |
| CT0001C | CAR Dextramer®, hCD19 | 50 tests | PE |
Want to see the results for yourself?
Our collaborators have tested the performance of CAR Dextramer® compared to other techniques for CAR-T cell detection. The results show that CAR Dextramer® (hCD19) is more effective than other staining reagents in detecting CAR-T cells with low CAR expression and offers superior assay robustness. Contact us and we will be happy to present these results to you.
Contact us
Looking for other targets than CD19? Contact us!
Enhanced CAR Cell Detection with Dextramer® Technology
- Optimized Reagent Design: our more than 10 years of expertise in immune monitoring allows us to optimally design your reagent for maximum detection performance
- Enhanced Sensitivity through Multimerization: Dextramer® enables multimerization of the target antigen, enhancing sensitivity in CAR detection, especially when CAR expression is low
- Reliable Results: Provides consistent, reproducible, and comparable results across experiments
- Easy staining protocol: Our stable, optimized reagents allow for staining at room temperature with minimal wash steps, easing your workflow and minimizing the handling of your cell samples
- Simultaneous phenotyping: Our reagents stain at room temperature, enabling simultaneous phenotypic analysis of surface markers, like CCR7 and CD45, for optimized workflow and minimizing sample use
Applications of Dextramer® Technology in CAR-T Cell Therapy
- Direct CAR cell detection
- Enhanced sensitivity for low-affinity CARs, due to the high avidity of Dextramer® technology and multiple fluorophores
- Assessment of transduction levels
- Determine the % of CAR-positive cells
- Demonstrate that the infusion product meets the defined lot release criteria
- Monitor kinetics and persistence of infused CAR cells in patient blood samples
- Deeper characterization of target specific CAR cells with gene and surface marker expression by single-cell multi-omics
FDA Guidance for CAR-T cell Therapy Products
The most recent guidance from the FDA regarding the development of CAR cell products includes recommendations for detecting and evaluating the CAR product.
Dextramer® reagents can assist you in adhering to this guidance.
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Dextramer® Controls
We recommend using negative controls with CAR Dextramer® reagents to accurately distinguish between background and antigen-specific staining, helping you optimize gating or sorting of positive cells.
Our recommended negative controls can be found here. If these don't meet your needs, contact us using the form at the bottom of this webpage, and we’ll create a custom solution for you.
Related Resources
CAR Application Brochure
Learn how Dextramer® technology may enable detection and characterization of CAR-T cells.