Custom CAR Dextramer®for Sensitive CAR Cell Detection and Characterization

Discover the power of CAR Dextramer® reagents for the sensitive detection and characterization of CAR-positive cells using flow cytometry.

CAR Dextramer® Reagents

CAR Dextramer® reagents enhance sensitivity, enabling the reliable detection of even lowly expressed CARs using a rapid one-step staining protocol.

Through multimerization of high-quality target proteins and fluorochromes, CAR Dextramer® provides researchers and clinicians with a highly precise and sensitive tool for CAR cell detection.

Read the Blog: “Avidity can make all the difference in detecting CAR-engineered cells”.

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How does it work?

Our expert team designs custom CAR Dextramer® reagents for you, based on your specific targets.

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Related Products

CAR Dextramer hCD19 is already available as an off-the-shelf reagent.

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New Targets Coming Soon!

BCMA | CD22 | HER2

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Want to see the results for yourself?

Our collaborators BioNTech SE have compared four CAR detection reagents across different constructs and expression levels.

  • The Results? CAR Dextramer® CD19 delivered strong detection in half the time
  • CAR Dextramer® CD19 enabled superior sensitivity for low-expressing CAR constructs
  • CAR Dextramer® bypasses scFv limitations like FMC63-only antibodies

Dive into the performance data in this blog post.

View performance data

Enhanced CAR Cell Detection with CAR Dextramer®

  • Optimized Reagent Design: Backed by over a decade of expertise in immune monitoring, our reagents are meticulously designed for maximum detection performance.
  • Enhanced Sensitivity through Multimerization: Dextramer® enables multimerization of the target antigen, enhancing sensitivity in CAR detection, especially when CAR expression is low.
  • Reliable Results: Provides consistent, high-quality results across experiments, ensuring reliable data for research and clinical applications.
  • Simplified Staining Protocol: Our stable, optimized reagents allow for staining at room temperature with minimal wash steps, easing your workflow and minimizing the handling of your cell samples.
  • Simultaneous phenotyping: Room-temperature staining enables the detection of CAR T cells alongside key surface markers, such as CCR7 and CD45, for efficient and comprehensive analysis.

Applications of CAR Dextramer® in CAR Cell Therapy

  • Direct CAR cell detection 
  • Functional assessment of CAR-antigen interactions 
  • Enhanced sensitivity for cells with low CAR expression due to the high avidity of Dextramer® technology and multiple fluorophores 
  • Determine the % of CAR-positive cells 
  • Demonstrate that the infusion product meets the defined lot release criteria 
  • Monitor kinetics and persistence of infused CAR cells in patient blood samples 
  • Deeper characterization of target-specific CAR cells with gene and surface marker expression by single-cell multi-omics 

FDA Guidance for CAR-T cell Therapy Products

The most recent guidance from the FDA regarding the development of CAR cell products includes recommendations for detecting and evaluating the CAR product.

Dextramer® reagents can assist you in adhering to this guidance.

Find the FDA guidance here

or

Learn more in our CAR-T Application Brochure

Dextramer® Controls

We recommend using negative controls with CAR Dextramer® reagents to accurately distinguish between background and antigen-specific staining, helping you optimize gating or sorting of positive cells.

One possible negative control Dextramer® reagent can be found here. Alternatively, we are happy to discuss other negative controls with you, or create a custom solution for you.

Related Resources

Cell Therapy

Explore how Dextramer® reagents support the development of effective cell therapies.

CAR-TCR Poster

Discover antigen-specific solutions for T-cell therapy and manufacturing.

CAR Therapy Brochure

Learn how CAR Dextramer® enables superior detection and characterization of CAR cells.

CAR Detection

Explore antigen multimers and other staining tools for CAR-T detection.

 

Questions? 

If you’re interested in exploring whether CAR Dextramer® could be beneficial for your CAR detection needs, please fill in the form and one of our dedicated specialists will get in touch with you shortly.